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In this review we focus on recent progress in protoplast regeneration, symmetric and asymmetric hybridization and novel technology developments. Disadvantages of Protoplast Fusion During the mechanical method of isolation of protoplasts: 1. It yields a very small amount of protoplasts. achievements and limitations of protoplast research pdf ppt on achievements of india in space research, achievement limitation of protoplast.

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Furthermore, nowadays significant efforts are made to establish high throughput bioassays to discover novel molecules with potent cytokinin activities, as exemplified by Motte et al.

A similar procedure is in use for the selection of other somatic hybrids e.

Somatic Hybridization: Aspects, Applications and Limitations

Although the genetic basis of the morphological characters has not been clearly known, intermediate morphological features suggest that the traits are under the control of multiple genes. Somatic hybridization is one of many breeding tools available to create various new genomic combinations, and is essentially different from other techniques in many respects.

Do and Zhanyuan J. Many a times, production of full-pledged hybrids through fusion of protoplasts of distantly related higher plant species is rather difficult due to instability of the two dissimilar genomes in a common cytoplasm. Genome fragmentation of the donor partner stimulates the elimination of liimitations of its redundant genetic material in the somatic hybrid.

Petunia parodii protoplasts species B form small colonies, but are resistant to actinomycin D.

A major advantage of embedding systems is the easy refreshment of the cultures. There are many enzymes possessing unique isoenzymes that can be used for the identification of somatic hybrids e.


Achievements and limitations of protoplast research pdf

The major limitation of this method is the requirement of specialized and costly equipment. The so isolated heterokaryons can be cloned to finally produce somatic hybrid plants.

In addition to further technical improvements, a better biochemical characterization of oxygen deficiency in protoplast culture could provide useful data for further breakthroughs.

The introduction of disease resistance into commercial poplar clones has been limited by interspecific incompatibility, but other solutions to the problem have been identified, e.

In fusogen or electro-induced fusions, about one third of the fusions occur between more than two protoplasts.

Isolation increases putrescine levels, especially in non-totipotent protoplasts Papadakis et al. There are many plants that cannot reproduce sexually. Freshly isolated protoplasts can be fused by induction. Advanced molecular screening methods of both genome and cytoplasmome facilitate efficient screening of both symmetric and asymmetric fusion products.

Sometimes, the hybrids are found to contain more chromosomes than the total of both the parents. Somatic hybridization has made it possible to transfer several desirable genetic characters among protoplsat plants Table Asymmetric hybrids have abnormal or wide variations in the chromosome number than the exact total of two species.

Another potential innovative tool for cell sorting is DePArray Silicon Biosystemsa new platform based on moving dielectrophoresis cages. It is based on chemically induced protoplast aggregation and direct current pulse promoted membrane fusion. Zhang Abstract Sorghum achievmeents is one of the most important crops.

Normally, cybrids are produced when protoplasts from two phytogenetically distinct species are fused.

Progress in plant protoplast research.

Lagging chromosomes could contribute to the formation of small pollen grains that are linked to sterility Guo et al. Fusion between protoplasts of potato Solanum tuberosum and tomato Lycopersicon esculentum has created pomato Solanopersicon, a new genus. The different levels of as well ROS and antioxidant enzymes and scavengers in Citrus callus and mesophyll are suggested to play a key role in defining the regeneration potential of protoplasts of both cell types Xu et al.


The presence of chromosomal markers is greatly useful for the genetic analysis of hybrid cells.

These fused cells may sometimes contain high number of nuclei Protoplasts of Lilium and Trillium in enzyme solutions can be fused by gentle trapping in a depression slide. Moreover, most karyotype instability causing donor genes are eliminated during the first post-fusion mitoses, as opposed to symmetrical researfh after which eliminations can occur up to the first sexually derived generation Cui et al.

When these two species are fused, the fused protoplasts derive both the characters — formation of macroscopic colonies and resistance to actinomycin D on MS medium.

The most important objective in Solanum tuberosum somatic breeding is the introduction of resistance against the PvY virus, Colorado beetle and late blight Phytophtora. Intergenomic chloroplast recombination occurs rarely in higher plants, as opposed to the high level of mitochondrial recombination Trabelsi et al.